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Product Info Summary
| SKU: | LS010534 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of Sjogren syndrome/scleroderma autoantigen 1 (SSSCA1) |
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Product info
Product Name
ZNRD2 (NM_006396) Human Over-expression Lysate
SKU/Catalog Number
LS010534
Size
100 µg
Description
Transient overexpression lysate of Sjogren syndrome/scleroderma autoantigen 1 (SSSCA1)
Storage & Handling
Cite This Product
ZNRD2 (NM_006396) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010534)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ZNRD2 Human Over-expression Lysates NM_006396.
Western blot validation of overexpression lysate (LS010534) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SSSCA1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ZNRD2 (Source: Uniprot.org, NCBI)
Gene Name
ZNRD2
Full Name
Protein ZNRD2
Weight
21.474kDa
Alternative Names
Protein ZNRD2 ZNRD2 SSSCA1, p27 zinc ribbon domain containing 2 protein ZNRD2|Sjogren syndrome/scleroderma autoantigen 1|Sjogrens syndrome/scleroderma autoantigen 1|autoantigen p27|centromeric autoantigen (27kD)|sjoegren syndrome/scleroderma autoantigen 1|zinc ribbon domain-containing protein 2
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ZNRD2, check out the ZNRD2 Infographic
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Specific Publications For ZNRD2 (NM_006396) Human Over-expression Lysate (LS010534)
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No publications found for LS010534
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